-Virtually eliminates use of antibodies.
-Extremely high specificity and sensitivity.
-No non-specific signaling and no background.
-Smaller than IgG.
-Does not require washing, blocking or fixing.
-Does not require colorimetric substrates.
-Eliminates need for radioactivity.

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Our split GFP Fold N’ Glow product is based on self-assembling GFP fragments that only glow when they come together. A properly folded protein can be expressed with a small GFP fragment attached to it. The small GFP fragment assembles with the larger GFP fragment to form a GFP fusion protein.

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The smaller fragment is small enough that does not interfere with folding of the protein of interest. In a misfolded protein, the smaller GFP fragment is obscured. This prevents self-assembly with the larger GFP fragment which in turn prevents the detection by fluorescence.

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The sGFP system is implemented by ligating a gene of interest between the 6HIS tag and the GFP fragment.

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S11 plasmid available for use in prokaryotic and eukaryotic systems. System provides fluorescent signals in green, yellow or cyan. More rapid, specific and cost-effective than antibody-based technologies. Can also be used to determine protein expression, folding, aggregation.

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-Rapid and efficient gain-of-fluorescence protease mapping tool
- Provides a highly stable fluorescent output signal.

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Split GFP technology users
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Protein Expression

1 Sandia Biotech’s split GFP and super-folder GFP systems can be used to quantify protein expression.  The split GFP products allow for a protein of interest to be generated with a small tag that does not interfere with protein conformation or function. The tagged protein can be detected and quantified with a probe that only fluoresces when bound to the tag.  For protein detection and quantitation in harsh conditions, the super-folder product will tag a  protein of interest and remain fluorescent in extreme temperatures and pH ranges.

Protein Folding

2 The small 16aa sGFP tag is placed on a protein fusion created with the use of our supplied plasmid. The tag is small enough that it will not interfere with the protein’s folding or functionality.  When a protein is mis-folded the small tag becomes conformationally obscured. The obscured tag will not complement with the probe and not fluoresce. Synthesized proteins can thus be screened for further research or production.

 Aggregation/      Solubility

3 Proteins that have aggregated will also obscure the sGFP tag and prevent complementation with the probe.  Successful disaggregation can be detected and quantified with the probe when the tag becomes available once more.  Proteins that have become insoluble will not be available to the soluble probe either and will thus complement in a limited manner and attenuate fluorescence.

Cellular               Localization

4 The sGFP probe is only 25 kDa, 1/5th the size of an IgG.  Accordingly, it can more easily traverse a permeated cell membrane or organelle membrane.  Once a tagged protein is secreted in a cell, the tag, due to its small size, will not interfere with the proteins function or become toxic.  These two attributes make the sGFP platform ideal for protein localization and trafficking in a cell.

 

Our Difference

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Cutting Edge Protein & Peptide Analysis Tools

GFP Green Florescent Protein & Modified Green Florescent Protein

Sandia Biotech, Inc. specializes in manufacturing protein and peptide analysis tools for the medical, drug development and industrial biotech communities.

Our focus is to expand, manufacture and market a Split Green Fluorescent Protein (sGFP) technology licensed from Los Alamos National Laboratory (LANL). We expect to gain universal acceptance as the detecting and tracking tool of choice for protein and peptide industry world-wide.

We currently manufacture and globally distibute three forms of sGFP technology that are packaged in ready-to-use kits: a prokaryotic sGFP detection system, a mammalian (with eukaryotic-optimized s11 gene) sGFP detection system, and a Superfolder™ ultra stable intact GFP system.

We also manufacture and market Quick Step ™, a rapid peptide analysis system. Sandia Biotech has an alliance American Peptide Company, a global leader in custom peptide synthesis.

Please contact us prior to ordering Quick Step™ with any peptide-specific or assay-specific inquiries.

Cyan and Yellow sGFP Detection Reagents Available.

Cyan Detection Reagent Cat # 21004004 (US$350) and Yellow Detection Reagent Cat # 21004005 (US$350)- These products represent cyan and yellow variants of the “Fold ‘n’ Glow” Split GFP Detection Reagent (Cat# 21004001). Either or both of these detection reagents can be used to detect proteins and peptides labeled with the Sandi Biotech, Inc. S11 tag. No new tag is required, and  these variants are compatible with both bacterial and mammalian split GFP detection systems. These detection reagents can be used in any flourimetric or imaging device that has the appropriate filters or imaging hardware. The use of the cyan and/or yellow detection reagent allows for differential staining and multiplexing assays where a color other than green is required.